Wales forwards Alun-Wyn Jones and Josh Turnbull have been ruled out of the remaining end-of-year Tests due to injuries.
Ospreys lock Jones (shoulder) and Scarlets flanker Turnbull (knee ligaments) will miss Friday's clash with Samoa, as well as matches against New Zealand and Australia.
The duo sustained the injuries during Wales' 26-12 loss to Argentina in Cardiff last weekend.
No timescale has been put on Jones' return but Turnbull is expected to be on the sidelines for four weeks.
Meanwhile, both flanker Ryan Jones and Ospreys' centre Ashley Beck have been fully re-integrated into training and should be available for selection for the Samoa Test.
Blues centre Jamie Roberts is continuing to be assessed for mild concussion and has a chance of facing Samoa.
There has been no change to the condition of Scarlets centre Jonathan Davies who is managing a groin problem and is currently unable to train fully with the squad.
No further call-ups have been made to the squad at this stage.