Italy head coach Jacques Brunel has named a familiar-looking squad of 30 players for the upcoming Six Nations.
Neither of the Bergamasco brothers have been included with place-kicking Mirco still recovering from knee surgery last November while Zebre flank Mauro has struggled to return to his best form since missing last year's Championship due to injury.
The squad will get together on January 20 for a training camp in Rome, with French head coach Brunel sticking with 27 players who featured last November.
The three newcomers are Paul Derbyshire and Gonzalo Garcia, who both last appeared in the 2011 Rugby World Cup, and Zebre winger Paolo Buso, who will get the chance to add to a solitary cap won back in 2008.
Stade Franšais number eight Sergio Parisse, who has 91 caps to his name, will continue to lead the side.
The Azzurri kick off their campaign by hosting France at the Olympic Stadium on February 3, and have two other home games, against Wales on February 23 and then Ireland on March 16.
Italy, who recently came close to securing an historic draw against Australia in the November tests, also play Scotland and England away.
Backs: Tommaso Benvenuti, Tobias Botes, Kristopher Burton, Paolo Buso, Gonzalo Canale, Gonzalo Garcia, Edoardo Gori, Tommaso Iannone, Andrea Masi, Luke McLean, Luciano Orquera, Alberto Sgarbi, Giovanbattista Venditti
Forwards: Roberto Barbieri, Martin Castrogiovanni, Lorenzo Cittadini, Alberto De Marchi, Paul Derbyshire, Simone Favaro, Joshua Furno, Quintin Geldenhuys, Leonardo Ghiraldini, Davide Giazzon, Andrea Lo Cicero, Francesco Minto, Sergio Parisse, Antonio Pavanello, Michele Rizzo, Ratu Manoa Vosawai, Alessandro Zanni